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Does anybody know when and how linguistic negation was manifested in proto-language (anyone, for example, proto-indo-european)?

What is meant by "linguistic negation" is these patterns of language that include negative references, i.e "not this", "not that".

References would be great as well. Thanks.

Update

The post by @TKR on Diachronic sources of negators approaches the spirit of the question, but it is not what is asked here. The question is about initial manifestation (and historical evolution) of negation in language (part of a greater study of negation, including linguistics)

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    There's no general answer to this question. Proto-languages are just languages like any others, so each one will do things differently.
    – TKR
    Sep 16, 2015 at 21:42
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    utexas.edu/cola/centers/lrc/books/pies04.html scroll down to 4.3.3 Sentence Negation
    – Alex B.
    Sep 17, 2015 at 1:18
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    Protolanguages are not real languages that exist and can be studied; they're reconstructed formulas that chart the best possible guesses for some parts of a protolanguage's vocabulary, morphology, and phonology, with many many rules for going from the protolanguage to various attested languages. But as far as we know, negation is present in every human language ever encountered. See here for more on the subject.
    – jlawler
    Sep 17, 2015 at 1:24
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    Related: linguistics.stackexchange.com/questions/2565/… The referenced documents there also may aid answering the question. Sep 17, 2015 at 13:58
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    See linguistics.stackexchange.com/questions/6571/…
    – TKR
    Sep 17, 2015 at 17:38

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